Abstract：The seismic inversion, which is one of the effective techniques for searching for sublle oil/gas traps, can enhance the resolution of seismic data greatly and extract abundant lithological and physical information involved in seismic data to the maximum. Simulated annealing wave impedance inversion pursues the comprehensive optimal solution widely in the space scope of the model, which can enhance the resolution of the inversion section markedly. The lithological reservoirs in the Dainan Formation in the Caozhuang area, Gaoyou subbasin, Subei basin, were predicted in detail by the ISIS3D simulated annealing wave impedance inversion technique. The results better describe the plan distribution characteristics of sands of three main lithological reservoirs in the Dainan Formation.