Abstract：For the purpose of detecting the formation condition, occurrence regularity and chemical characteristics as well as the recharge, runoff and drainage characteristics of groundwater, the building of monitoring well for sample collection is necessary. This paper deals with construction materials, well completion technology and sampling techniques, and presents the optimization of PVC-U well pipe and gravel filter as well as the differential shut of water and stratified sampling of monitoring well in the endemic disease area. Typical groundwater samples are collected through the depth preset sampling and double-packer systems.