The Yangchuling tungsten-molybdenum deposit in Duchang County, Jiangxi Province, is located in the eastern part of the Jiangnan orogenic belt. In order to accurately define the diagenetic and mineralization ages of the Yangchuling W-Mo deposit, the authors carried out the zircon U -Pb dating of mineralization -associated granodiorite porphyry and Re -Os isotope dating of molybdenite in the Yangchuling deposit based on detailed field geological studies. The zircon LA-ICP-MS U-Pb age is (145.08±0.35)Ma (MSWD=0.03, n=22), the Re-Os model ages of molybdenite range from (143.3±2.0)Ma to (145.5±2.2)Ma, and the Re-Os isochron age is (145.4±1.0)Ma. The geochronological consistency between the ore-bearing porphyry and molybdenite indicates that the diagenesis and mineralization ages of the Yangchuling tungsten-molybdenum deposit are about 145 Ma. The ω(Re) values of molybdenite range from 16.62×10^-6 to 87.76×10^-6, averaging 44.68×10^-6, similar to Re content in crust -mantle mixed source magmatic hydrothermal deposits, indicating that the ore-forming materials of the Yangchuling W-Mo deposit should have come from crust-mantle mixed source.