This paper is the result of oil and gas exploration engineering.[Objective] The oil and gas resources of Yan'an Formation in Wuqi area of Ordos Basin are widely distributed and have great exploration potential. The systematic study on the accumulation time and stages of Yan10 reservoir of Yan'an Formation in Wuqi area is beneficial to the fine oil and gas exploration and development process, and provides a basis for understanding and perfecting the nature of Jurassic hydrocarbon accumulation in the whole basin. [Methods] In this paper, the characteristics of reservoir fluid inclusions in the study area were analyzed by means of microscopic observation and indirect projection dating of fluid inclusions homogenization temperature and burial history, so as to further determine the hydrocarbon accumulation period. [Results] The early and late inclusions exist in Yan10 reservoir of Jurassic Yan'an Formation in Wuqi area of Yishan slope, Ordos Basin. The early fluid inclusions are mainly distributed in dissolution cracks and quartz secondary enlarging edges on the surface of quartz grains; The late fluid inclusions are mainly distributed in the fractures and the surface of quartz particles, while they are distributed sporadically in the late siliceous cements and calcite cements. The peak homogenization temperature of early inclusions is 80-90℃. The peak of homogenization temperature of late inclusions is 100-110℃, and the homogenization temperature of the two phases is continuously distributed. [Conclusions] There are two consecutive hydrocarbon charging periods in Yan10 reservoir of Yan'an Formation in Wuqi area of Yishan slope, Ordos Basin: the first oil and gas charging period corresponds to 112-106 Ma. The corresponding time of the second oil and gas charging is about 102-97 Ma. Two oil and gas charging shows that the oil and gas accumulation period in the study area is 112-97 Ma ago, which is the end of the Early Cretaceous.