Abstract：Daning batholith, located in the border area between Yangtze plate and Cathaysia plate, consists mainly of monzodiorite,granodiorite and monzogranite with minor granite. In this paper, zircon SHRIMP U-Pb dating and geochemical characteristics of Daning batholith are reported to discuss the magma source and genesis of this Caledonian intrusion in northern Guangxi of South China. Zircon SHRIMP U-Pb dating of the late stage granite yielded an age of （419.1±6.4） Ma（MSWD=1.03）, which provides the upper constraint of the batholith formation in Late Caledonian. The major element compositions show that the granites are weakly peraluminous and strongly potassic-calc-alkaline. Geochemical characteristics of the granites indicate that this pluton was derived from partial melting of metamorphosed feldspathic clastic lower crust. The granites are commonly enriched with large ion lithophile elements and light rare earth elements with high Rb/Sr and low Nb/Ta ratios, showing behaviors of high Sr and low Nd isotopic combination. However, TDM of the granites are obviously lower than the average value of normal granite in this area, which is consistent with the regional linear distribution of lower TDM of Caledonian granites. Combined with field observation of widely spread mafic microgranular enclaves in the batholith, the authors consider that an intense mantle-crust interaction probably occurred during the anatectic event in Late Caledonian. Mantle constituents were obviously involved in the formation of anatectite.