全文快速搜索:          
引用本文:何泽,宁卓,黄冠星,刘丹丹,张千千,孙继朝. 太行山前平原浅层地下水污染的分子生物学响应特征——以滹沱河流域为例[J]. 中国地质, 2019, 46(2): 290-301.
HE Ze,NING Zhuo,HUANG Guanxing,LIU Dandan,ZHANG Qianqian,SUN Jichao. The response characteristics of microbial diversity to shallow groundwater contamination in the piedmont of the Taihang Mountains using molecular biotechnologies: A case study of groundwater of Hutuo River Basin[J]. Geology in China, 2019, 46(2): 290-301(in Chinese with English abstract).
【打印本页】   【HTML】   【下载PDF全文】   查看/发表评论  下载PDF阅读器  关闭
←前一篇|后一篇→ 过刊浏览    高级检索
本文已被:浏览 168次   下载 213 本文二维码信息
码上扫一扫!
分享到: 微信 更多
太行山前平原浅层地下水污染的分子生物学响应特征——以滹沱河流域为例
何泽1,2, 宁卓1,2, 黄冠星1,2, 刘丹丹3, 张千千1,2, 孙继朝1,2
1.中国地质科学院水文地质环境地质研究所, 河北 石家庄 050061;2.中国地质调查局/河北省地下水污染机理与修复重点实验室, 河北 石家庄 050061;3.中国地质调查局水文地质环境地质调查中心, 河北 保定 071051
摘要:
分子生物学技术是地下水污染研究的前沿技术,常用于场地尺度,区域尺度罕见。以太行山前平原滹沱河流域的典型浅层地下水为研究对象,沿河布点采样67组,采用高通量测序技术,测定样品16S rDNA基因序列,以化学需氧量、硝酸盐、溶解性总固体为环境因子,分析与污染相关的微生物种群结构响应及功能性指示菌属。结果显示:采用累积概率分布法将样品分为背景(B)、硝酸盐污染(N)、有机污染(Y)3组,该分类阈值与地下水质量标准的Ⅲ类水阈值可较好对应;微生物群落丰富度为B组 > N组 > Y组,有机污染使微生物种群趋于单一,且与背景差异更大。有机污染功能性指示菌属为Acinetobacter,硝酸盐污染为Nitrospira。以上形成的分子生物学响应特征研究方法可为区域调查及修复提供技术方法理论依据。
关键词:  滹沱河  浅层地下水  硝酸盐污染  有机污染  分子生物学
DOI:10.12029/gc20190206
分类号:
基金项目:中国地质调查局地质调查项目(DD20160309)、国家自然科学基金资助项目(41602261)、中国地质科学院基本科研业务费专项经费(SK201702)及河北省自然科学基金资助项目(D2016504021)联合资助。
The response characteristics of microbial diversity to shallow groundwater contamination in the piedmont of the Taihang Mountains using molecular biotechnologies: A case study of groundwater of Hutuo River Basin
HE Ze1,2, NING Zhuo1,2, HUANG Guanxing1,2, LIU Dandan3, ZHANG Qianqian1,2, SUN Jichao1,2
1.Institute of Hydrogeology and Environmental Geology, Chinese Academy of Geological Sciences, Shijiazhuang 050061, China;2.Key Laboratory of Groundwater Remediation, China Geological Survey, Hebei, Shijiazhuang 050061, China;3.Center for Hydrogeology and Environmental Geology, China Geological Survey, Baoding 071051, Hebei, China
Abstract:
In the groundwater contamination research, molecular biotechnologies are one of the cutting-edge technologies. This kind of technologies have been commonly used at site scale, but rarely applied at regional scale. The shallow groundwater in Hutuo River Basin was selected as the study subject, because of it is one of the typical regions in front of the Taihang Mountains. 67 samples were collected along the river. The 16s rDNA gene sequences were tested by high-throughput sequencing technology. According to the environmental factors of NO3-, COD and TDS, the microbial communities and the functional indicator microorganisms related to groundwater contamination were studied. The results showed that, using the cumulative probability distribution method, the samples were divided into three groups:background (B group), nitrate pollution (N group), and organic pollution (Y group). This grouping rule was close to the Ⅲ water grade threshold in the quality standard of groundwater. The order of microbial community richness is B group > N group > Y group. The organic pollution could decrease the richness diversity of microbial communities, and the difference was more significant in comparison with background microorganisms. The functional indicator microorganisms related to organic pollution was Acinetobacter, and the functional indicator microorganisms related to nitrate pollution was Nitrospira. The molecular biotechnologies and analysis methods used in this research could provide the theoretical evidence for regional environmental investigation and bioremediation.
Key words:  Hutuo River basin  shallow pore groundwater  nitrate pollution  organic pollution  molecular biotechnologies